食品科学2017，Vol.38Issue(6)：147-152,6.DOI:10.7506/spkx1002-6630-201706023
响应面试验优化杏鲍菇液体发酵菌种培养条件
Optimization of Culture Conditions for Liquid-State Fermentation of Pleurotus eryngii by Response Surface Methodology
摘要
Abstract
In this work,a three-level Box-Behnken factorial design was employed to optimize the culture conditions for liquid-state fermentation of Pleurotus eryngii.By the combined use of one-factor-at-a-time method and response surface methodology,the effect of four different factors on dry mycelial biomass was determined to be in the order of rotation speed ＞ initial pH ＞ inoculum quantity ＞ medium volume and their optimal levels were 147.00 r/min,5.90,9.50％ and 95.00 mL/250 mL,respectively.Under these conditions,the dry mycelial biomass was 1.265 7 g/100 mL.In conclusion,this study will provide evidence for the cultivation and bioactive components analysis of P.eryngii.关键词
杏鲍菇/液体菌种/发酵条件/响应面分析Key words
Pleurotus eryngii/liquid starter culture/fermentation conditions/response surface methodology分类
生物科学引用本文复制引用
张杰,侯潞丹,贺志斌..响应面试验优化杏鲍菇液体发酵菌种培养条件[J].食品科学,2017,38(6):147-152,6.基金项目
山西师范大学科技开发基金项目(YK1201) （YK1201）
