食品科学2017，Vol.38Issue(6)：153-159,7.DOI:10.7506/spkx1002-6630-201706024
包埋培养法筛选白酒窖泥中一株功能细菌
Screening of a Functional Bacterial Strain from Chinese Liquor Pit Mud by Microencapsulation Culture Method
摘要
Abstract
A total of 95 bacterial strains were isolated from Chinese liquor pit mud by the microencapsulation culture method through cell sorting using flow cytometry.Out of these isolates,the strains LS4,LS27,LS32,LS37,LS64,LS66,LS69'and LS85 were selected for slow growth rates.Gas chromatography analysis demonstrated that LS66 was determined as the best one for the production of an abundance of aroma compounds in Chinese liquor.By morphological observation,physiological and biochemical tests and 16S rDNA sequence analysis,it was tentatively identified as an uncultured functional bacterium,termed N8-7.The optimal culture temperature,pH and inoculums size for this strain were determined to be 35 ℃,6.5 and 4％,respectively.关键词
白酒/窖泥/细菌/微包埋/未培养Key words
liquor/pit mud/bacteria/microencapsulation/uncultured分类
生物科学引用本文复制引用
宋连宝,赵辉..包埋培养法筛选白酒窖泥中一株功能细菌[J].食品科学,2017,38(6):153-159,7.基金项目
黑龙江省博士后科研启动基金项目(LBH-Q13139) （LBH-Q13139）
