食品科学2017，Vol.38Issue(6)：179-184,6.DOI:10.7506/spkx1002-6630-201706028
青稞麸皮提取物抑制α-葡萄糖苷酶活性研究及成分分析
Anti-α-Glucosidase Activities and Bioactive Components of Tibetan Hull-Less Barley Bran Extracts
摘要
Abstract
The hexane,ethyl acetate and methanol extracts of Tibetan hull-less barley (THB) bran displayed dose-dependent inhibitory activities on α-glucosidase in vitro.The hexane and ethyl acetate extracts had higher inhibitory activities than did acarbose,which is used in clinical treatment of type 2 diabetes.Gas chromatography-mass spectrometry (GC-MS) analysis showed that linoleic acid,oleic acid,palmitic acid and α-linoleic acid were the main fatty acids in the hexane extracts,accounting for 96.5％ of the total fatty acids.All of these four fatty acids have been reported to be potent inhibitors of α-glucosidase.Moreover,65 peaks were separated from the unsaponifiable fractions,with 17 of them identified as sterols,triterpenes and long chain fatty alcohols,respectively.The total free phtosterol content was 7 357.72 mg/kg,which presented β-sitosterol,campesterol,desmosterol,stigmasterol,and fucosterol as the primary phytosterols.In addition,β-sitosterol and campesterol accounted for 83％ of the total phtosterols.These results show that THB can be a natural source of potent α-glucosidase inhibitors.关键词
青稞麸皮/α-葡萄糖苷酶抑制剂/气相色谱-质谱联用/脂肪酸/不皂化物Key words
Tibetan hull-less barley bran/α-glucosidase inhibitors/gas chromatography-mass spectrometry (GC-MS)/fatty acid/unsaponifiable matters分类
轻工纺织引用本文复制引用
龚凌霄,曹文燕,张英,张慧娟,刘英丽,王静..青稞麸皮提取物抑制α-葡萄糖苷酶活性研究及成分分析[J].食品科学,2017,38(6):179-184,6.基金项目
国家自然科学基金青年科学基金项目(31501480) （31501480）
