食品与机械2016，Vol.32Issue(10)：44-47,62,5.DOI:10.13652/j.issn.1003-5788.2016.10.010
基于多肽识别基团的荧光探针及其对食品中氰化物的检测
Devise and synthesis of fluorescent probe for cyanide detection in food based on a peptide receptor
摘要
Abstract
Cyanide is considered to be one of the most toxic species in food while the existing detection methods involve complex operation and will produce toxicity to inspectors.In this study,a water-soluble fluorescent probe(C-GGH) was synthesized by HOBT/DIC protocol in solution using 7 diethylaminocoumarin-3-carboxylic acid,Glycine and Histidine.Due to the presence of the recognition unit GGH,the probe C-GGH can conjugated with Cu2+ to formed non-fluorescent C-GGH-Cu2+complex.Due to the strong binding affinity of CN-to Cu2+,CN-can extract Cuz+ from C—GGH-Cu2+ complex,leading to the release of C-GGH and the recovery of fluorescent emission of the system.The probe allowed detection of cyanide in aqueous solution with a LOD (limit of detection) of 0.017 μmol/L which is much lower than the maximum contaminant level (0.385 μmol/L)for cyanide in mineral water set by national standard (GB 8537-2008).The probe also displayed excellent specificity for CN-towards other anions,including F-,Cl-,Br-,I-,SCN-,PO43-,N3-,NO3,AcO-,SO4242-,and CO32-.The method developed in this study received the same results as national standard method (GB/T 5009.48-2003,GB/T 5009.36-2003,GB/T 8538-2008),but more simple and quick in real sample detection.关键词
食品/氰化物/多肽/荧光探针/检测Key words
food/cyanide/peptide/fluorescent probe/detection引用本文复制引用
周彬彬,汪霞丽,王芳斌,张继红,杨滔,程云辉,郝远强..基于多肽识别基团的荧光探针及其对食品中氰化物的检测[J].食品与机械,2016,32(10):44-47,62,5.基金项目
国家自然科学基金项目(编号:U1404215,31171627,31071523) （编号:U1404215,31171627,31071523）
湖南省食品药品监督管理局食品药品安全科技项目(编号:湘食药科R201516) （编号:湘食药科R201516）
