食品与机械2016，Vol.32Issue(10)：151-157,7.DOI:10.13652/j.issn.1003-5788.2016.10.035
羊肺蛋白酶解工艺优化及体外抗氧化活性研究
Optimization of protease enzymatic hydrolysis of sheep lung and studies on antioxidant activities of its hydrolysates
摘要
Abstract
By using the hydrolysis degree as the evaluation,the sheep lung enzymatic process was optimized to investigate the antioxidate activities of the enzymolysis products.hydrolyzed by neutral protease.On the basis of single factor studies,the response surface analysis (RSM) assay was then used to obtain the best enzymatic proscess.Our results showed that the optimal condition was as follows.The enzyme amount was 1 700.0 U/g,with solid-liquid ratio 2 ∶ 1(mL/g),enzymatically hydrolyzed for 4 h at 50 ℃.Under this optimal conditions,3 times of the replication experiments turned out that the error of the actual degree of hydrolysis (18.89 ％) was small,compared with the theory one (18.19 ％).Moreover,the absorption value of reducing power was found to be 0.472,and IC50 of the DPPH radical scavenging rate,the superoxide anion radical scavenging rate,and the hydroxyl radical scavenging rate were 67 mg/mL,40.07 mg/mL,and 31.88 mg/mL,respectively.These results of above detection indicated that enzymatic hydrolysates of sheep lungs possessed certain antioxidant activity in virto.关键词
羊肺/蛋白/酶解/抗氧化Key words
sheep lung/protein/hydrolysis/antioxidant引用本文复制引用
杨旭卉,文飞,王先倩,齐琦,苏伟,托尼..羊肺蛋白酶解工艺优化及体外抗氧化活性研究[J].食品与机械,2016,32(10):151-157,7.基金项目
黔西南州种草养羊产业发展省州科技合作专项项目(编号:黔西南科号[2012]4) （编号:黔西南科号[2012]4）
