食品与机械2017，Vol.33Issue(2)：11-16,6.DOI:10.13652/j.issn.1003-5788.2017.02.003
兔皮胶原蛋白的微观结构及不同因素对其聚集特性的影响
Microstructure of rabbit-skin collagen and influences of different factors on its aggregation
摘要
Abstract
In this study,UV spectroscopy,Fourier transform infrared spectroscopy and scanning electron microscopy,were used to study the microstructure of rabbit-skin collagen,respectively.The effects of temperature,collagen concentration,pH value and ionic strength on aggregation properties of collage were also studied.The results of UV spectroscopy and Fourier transform infrared spectroscopy showed that the absorption peak and the corresponding wavelength in accordance with the characteristics of type Ⅰ collagen.Moreover,the results of scanning electron microscopy showed that the collagen was irregular dense sheet film,partially wrinkled on the surface.Furthermore,the results of aggregation properties of rabbit-skin collagen indicated that with the increase of temperature and collagen concentration,the aggregation speed of collagen accelerated and the degree of aggregation increased.In acidic environment,the aggregation time was relatively long.When pH increased from 7 to 8,the degree of self-assembly increased first and then decreased.The degree and the speed of self-assembly were the highest at pH 7.2.With the increase of ion concentration,the aggregation rate first increased and then decreased.The collagen reached the fastest aggregation speed and the highest degree of self-assembly in 120 mmol/L NaC1 solution.关键词
胶原蛋白/紫外光谱/红外光谱/扫描电镜/聚集特性Key words
collagen/UV spectroscopy/fourier transform infrared spectroscopy (FTIR)/scanning electron microscope/aggregation property引用本文复制引用
马明思,张宇昊,马良,王雪蒙,韩霜,张晓洁..兔皮胶原蛋白的微观结构及不同因素对其聚集特性的影响[J].食品与机械,2017,33(2):11-16,6.基金项目
国家自然科学基金项目(编号:31301425) （编号:31301425）
中央高校基本科研业务费重大项目(编号:XDJK2015A015) （编号:XDJK2015A015）
中国博士后科学基金面上项目(编号:2014M562267) （编号:2014M562267）
中国博士后科学基金特别资助项目(编号:2015T80951) （编号:2015T80951）
重庆市基础科学与前沿技术研究重点项目(编号:cstc2015jcyjBX0116) （编号:cstc2015jcyjBX0116）
