食品与机械2017，Vol.33Issue(2)：51-55,5.DOI:10.13652/j.issn.1003-5788.2017.02.011
高灵敏黄曲霉毒素B1免疫层析定量检测法的建立
Development of Quantitative Sensitive Immunochromatographic Assays for Detection of Aflatoxin B1
摘要
Abstract
The aim of this work was to establish a highly sensitive quantitative colloidal gold immunochromatographic assay with a test strip reader for the detection of aflatoxin B1 using colloidal gold as label.When the labeling pH was 6.0,the concentration of antigen on T-line was 1.5 mg/mL,the concentration of antibody labeled on the colloidal gold was 4 μg/mL,and the dosage of gold-labeled antibody was 1.2 μL,the sensitivity of the assay was 5.7 ng/L.The results of accelerated aging experiment indicated that the strips shelf time was more than a year.It was proved that this method can meet the needs of the rapid detection of food when the strip was applied to detect cereals and soybean sauce.关键词
AFB1/免疫层析/胶体金/加标回收Key words
AFB1/immunochromatographic assay/colloidal gold/recovery rate引用本文复制引用
刘静,王树颖,刘道峰,赖卫华..高灵敏黄曲霉毒素B1免疫层析定量检测法的建立[J].食品与机械,2017,33(2):51-55,5.基金项目
江西省主要学科学术和技术带头人培养计划项目(编号:20113BCB22007) （编号:20113BCB22007）
江西省教育厅落地项目(编号:KJLD13009) （编号:KJLD13009）
