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普鲁兰酶法制备缓慢消化淀粉工艺的研究

王磊 陈宇飞 杨柳

中国食品添加剂Issue(1):146-151,6.
中国食品添加剂Issue(1):146-151,6.

普鲁兰酶法制备缓慢消化淀粉工艺的研究

Study on preparation of slowly digestible starch by Pullulanase

王磊 1陈宇飞 2杨柳2

作者信息

  • 1. 吉林工商学院粮油食品深加工吉林省高校重点实验室,长春130507
  • 2. 吉林工商学院食品工程学院,长春130507
  • 折叠

摘要

Abstract

Using potato starch as raw material,pullulanase was chosen to prepare slowly digestible starch (SDS).The effects of enzyme amount,enzynolysis time,concentration of starch and refrigeration time were investigated by single factor tests.Subsequently,Box-Behnken design was used to investigate the effects of four factors on SDS mass concentration.The optimum combination was obtained by Design Expert 8.05 software and response surface analysis.The results showed that the optimum process conditions were as follows:enzyme amount 160U/mL,enzynolysis time 8.5h,concentration of starch 10%,refrigeration time 3d.Under the above optimized conditions,the mass concentration of SDS was 18.3%.The results provided a theoretical basis for the development of slowly digestible starch.

关键词

普鲁兰酶/马铃薯淀粉/缓慢消化淀粉

Key words

pullulanase/potato starch/slowly digestible starch

分类

生物科学

引用本文复制引用

王磊,陈宇飞,杨柳..普鲁兰酶法制备缓慢消化淀粉工艺的研究[J].中国食品添加剂,2017,(1):146-151,6.

基金项目

粮油食品深加工吉林省高校重点实验室科研项目(吉高校重点实验室科合字[2016]第017号). (吉高校重点实验室科合字[2016]第017号)

中国食品添加剂

OACSTPCD

1006-2513

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