中国食品添加剂Issue(1)：169-172,4.
固相萃取-反相高效液相色谱法测定汤料中的碱性橙Ⅱ
Determination of basic orange Ⅱ in soup stock by solid-phase extraction coupled with reversed-phase high performance liquid chromatography
陈伟 1乔勇升 1武嫱2
作者信息
- 1. 泰州市食品药品检验所,泰州225300
- 2. 上海安谱实验科技股份有限公司,上海201608
- 折叠
摘要
Abstract
A rapid and effective method was established for the determination of basic orange Ⅱ in soup stock by solid-phase extraction coupled with reversed-phase high performance liquid chromatography (SPE-RP-HPLC).The samples were extracted with acidified methanol solution.The supernatant was added to the HLB solid phase extraction cartridge,leaching with formic acid aqueous solution,eluting with ammoniated methanol.The solution was then filtrated by 0.22 μ m membrane before testing.The separation was performed by a Waters RP C18 chromatographic column (4.6mm*250mm,5 μ m) using methanol-ammonium acetate (20mmol/L) as mobile phase,with diode array detector and external standard method peak area quantification.The linear range of basic orange Ⅱ was in the range of 1.0 μ g/mL ～ 25.0 μ g/mL with a correlation coefficient of 0.9992.The detection limit was 0.04 μ g/mL and the quantitative limit was 0.12 μ g/mL.The recovery rate was 89.2％ ～ 91.5％.The method has the advantages of simple pretreatment,good purifying effect,high sensitivity and fast detection rate.It is suitable for the separation and quantitative detection of basic orange Ⅱ in soup stock.关键词
反相高效液相色谱/汤料/碱性橙ⅡKey words
reversed-phase high performance liquid chromatography (RP-HPLC)/soup stock/basic orange Ⅱ分类
轻工纺织引用本文复制引用
陈伟,乔勇升,武嫱..固相萃取-反相高效液相色谱法测定汤料中的碱性橙Ⅱ[J].中国食品添加剂,2017,(1):169-172,4.
