中国食品添加剂Issue(3)：122-126,5.
固定化荧光假单胞菌脂肪酶催化合成乙酸癸酯
Synthesis of decyl acetate catalyzed by immobilized Pseudomonas fluorescens lipase
摘要
Abstract
Decyl acetate is a food flavor enhancer,using environmental friendly,low-carbon and efficient synthesis method is an ideal way to obtain high purity decyl acetate.In this study,Pseudomonasfluorescens lipase (PFL) was a biocatalyst,CMC carboxymethyl cellulose was a regulator.FEL was immobilized on the inner wall of the reactor by physical absorbance.After 28 h,it was showed that the conversion rate was 98％;and PFL enzyme catalyzed reaction was only 76％ of conversion rate.If the system was enlarged to 50 times,CMC-PFL conversion rate was 99.4％ after 96h.The yield of decyl acetate was about 94.6 ％ by rotary evaporating.关键词
乙酸癸酯/脂肪酶/固定化/催化活性/非水相稳定性Key words
decyl acetate/lipase/immobilization/catalytic activity/non aqueous phase stability分类
化学化工引用本文复制引用
丁俊竹,丛方地,詹美榕,康洁,周文礼,邢克智..固定化荧光假单胞菌脂肪酶催化合成乙酸癸酯[J].中国食品添加剂,2017,(3):122-126,5.基金项目
天津市水产生态及养殖重点实验室开放基金项目(TJAE201502) （TJAE201502）
国家自然科学基金项目(31270456). （31270456）
