现代食品科技2017，Vol.33Issue(3)：8-13,34,7.DOI:10.13982/j.mfst.1673-9078.2017.3.002
马尾松花粉提取物对前列腺肥大小鼠的预防作用及代谢组学研究
Metabolomics Study of Pinus massoniana Pollen Extract and its Preventive Effect on Benign Prostate Hypertrophy in Mice
马玉 1杨长军 2孙丰霞 1吕建云 1王桐 2耿越1
作者信息
- 1. 山东省动物抗性生物学重点实验室,山东师范大学生命科学学院,山东济南250014
- 2. 烟台新时代健康产业(集团)有限公司,山东烟台264006
- 折叠
摘要
Abstract
The effect of masson pine (Pinus massoniana) pollen extract (MPPE) on benign prostate hypertrophy (BPH) in mice was studied.Male KM mice were randomly divided into six groups:normal control group,BPH model group,20,4080 mg/kg MPPE groups and 0.8 mg/(kg·d) finasteride group.After two weeks of advanced intragastric MPPE administration,an intraperitoneal injection of testosterone propionate was used to initiate the BPH,following which the MPPE or finasteride treatment was administered intragastically for 4 weeks.At the end of the treatment period,morphological changes in the tissues were observed,and levels of the cytokines IL-6,IL-lβ,and TNF-α as well as of PACP,DHT,NO,and NOS were measured.Ultraperformance liquid chromatography-mass spectrometry was employed to detect changes in serum and urine metabolites.Partial least squares discriminant analysis was used for group differentiation and biomarker selection.All three doses of MPPE decreased the prostate index,inflammatory cytokines,DHT concentration,PACP and NOS activities,and NO production.Serum metabolomics analysis found and identified three potential biomarkers:1-palmitoyl-sn-glycero-3-phosphocholine,1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine,and cis-13-docosenoamide.MPPE could significantly reduce the pathological symptoms and biochemical parameters of BPH and adjust the level of the marker metabolites.The mechanism may be related to the inhibition of inflammation and alleviation of lipid metabolism disorders.关键词
马尾松花粉提取物/良性前列腺肥大/代谢组学/炎症反应/氧化应激Key words
masson pine pollen extract/benign prostate hypertrophy/metabolomics/inflammatory response/oxidative stress引用本文复制引用
马玉,杨长军,孙丰霞,吕建云,王桐,耿越..马尾松花粉提取物对前列腺肥大小鼠的预防作用及代谢组学研究[J].现代食品科技,2017,33(3):8-13,34,7.
