现代食品科技2017，Vol.33Issue(3)：14-21,8.DOI:10.13982/j.mfst.1673-9078.2017.3.003
植物乳杆菌35对大肠杆菌在肠上皮细胞HT-29上黏附及刺激产生促炎细胞因子的影响
Effect of Lactobacillus plantarum 35 on Escherichia coli-induced IL-8 Production and E.coli Adhesion on HT-29 Cells
摘要
Abstract
The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line,and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides (EPS) on Escherichia coli-induced IL-8 production and E.coli adhesion on HT-29 cells were investigated.The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees,and L.plantarum 35 had the highest adhesive ability.L.plantarum 35 could protect HT-29 cells from E.coli adhesion by displacement,competition,and exclusion,with inhibition rates of 30％,33％,and 59％,respectively.At an active concentration of 500 μg/mL,EPS could inhibit E.coli adhesion by 32％.L.plantarum 35 could inhibit E.coli-induced IL-8 production by HT-29 cells by displacement,competition,and exclusion with inhibition rates of 3％,28％,and 40％,respectively.EPS could inhibit E.coli-induced IL-8 production by HT-29 cells in a dose dependent manner,with the highest inhibitory rate of 50％ at 500 μg/mL concentration.L.plantarum 35 had no effect on IL-10 production by HT-29 cells.The results showed that L.plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E.coli.关键词
乳酸杆菌/HT-29/大肠杆菌/黏附Key words
Lactobacillus/HT-29 cells/E.coli ATCC25922/adhesion引用本文复制引用
郭远骥,妥彦峰,牟光庆,蒋淑娟,钱方,刘鹏,盛晓芳..植物乳杆菌35对大肠杆菌在肠上皮细胞HT-29上黏附及刺激产生促炎细胞因子的影响[J].现代食品科技,2017,33(3):14-21,8.基金项目
国家十二五科技支撑计划项目(2013BAD18B09) （2013BAD18B09）
国家自然科学基金项目(31571813) （31571813）
国家海洋食品工程技术研究中心项目(2012FU125X03) （2012FU125X03）
辽宁省自然科学基金项目(2014026018)资助 （2014026018）
