现代食品科技2017，Vol.33Issue(3)：47-53,66,8.DOI:10.13982/j.mfst.1673-9078.2017.3.008
木犀草素配合物的制备表征及抑制黄嘌呤氧化酶的研究
Synthesis and Characterization of Luteolin Complexes and Their Inhibition Effects on Xanthine Oxidase
摘要
Abstract
The coordination effect was used to synthesize luteolin complexes between luteolin and metal ions Zn2+ and Mn2+,and the complexes were examined by ultraviolet UV-visible spectroscopy,infrared spectroscopy,thermogravimetric-differential scanning calorimetry,and elemental analysis techniques.Moreover,the inhibition of xanthine oxidase by the luteolin complex was studied,the inhibitory capacities of luteolin and its complexes were compared,and the type of inhibition ofxanthine oxidase was determined.The results indicated that coordination reactions occurred between luteolin and the metal ions Zn2+ and Mn2+ to form stable complexes.The chelation site was located at the 5-OH of ring A and 4-C=O of ring C.Compared to luteolin,its complexes exhibited a better inhibitory effect on xanthine oxidase.When the xanthine substrate concentration was 0.6 mM,the graph of enzyme concentration versus enmzyme activity showed that the types of inhibition by luteolin and its complexes were reversible.When the concentration of xanthine oxidase was 10 μg/mL,the double reciprocal Lineweaver-Burk plot showed that the types of inhibition by luteolin and its complexes were competitive.关键词
木犀草素/配合物/黄嘌呤氧化酶/抑制类型Key words
luteolin/complex/xanthine oxidase/type of inhibition引用本文复制引用
肖凯军,钟炼军,蔡胜,朱良,龚斌,朱子沛..木犀草素配合物的制备表征及抑制黄嘌呤氧化酶的研究[J].现代食品科技,2017,33(3):47-53,66,8.基金项目
国家自然科学基金项目(31371743) （31371743）
广州市产学研协同创新重大专项项目(201508020086) （201508020086）
广东省应用型科技研发专项资金项目(2015B020230001) （2015B020230001）
