现代食品科技2017，Vol.33Issue(3)：54-59,6.DOI:10.13982/j.mfst.1673-9078.2017.3.009
双重营养缺陷型蛹虫草突变株的选育及其高产虫草素的研究
Screening of a Double Auxotrophic Cordyceps militaris Mutant and Its High Fermentation Yield of Cordycepin
摘要
Abstract
Cordycepin,with a variety of medical and health benefits,is one of the key bioactive compounds in Cordyceps militaris.However,the low fermentation yield restricts its industrial production and applications.To enhance the yield of cordycepin,a xanthine-guanine double auxotrophic mutant,xan-gua-c,was obtained by ultrasound mutagenesis combined with diethyl sulfate treatments,followed by directional sceening,and its yield of cordycepin was improved to 1.76 g/L on the 20th day of batch fermentation,about 40％ higher than that of the control.The effects of structural analogs of cordycepin on the cordycepin production capacity of the mutant were then investigated.The results showed that the addition of 1.0 g/L adenosine and 1.0 g/L adenine could improve the production of cordycepin (on the 20th day) by 33.5％ (to 2.35 g/L) and 103.5％ (to 3.59 g/L),respectively,compared with that of the control.Metabolic pathway analysis indicated that there might be two metabolic pathways used to synthesize cordycepin from adenine or adenosine.The two metabolic processes might overlap,and blocking the anabolism of xanthine and guanine might be an effective strategy to promote Cordyceps militaris to accumulate cordycepin.关键词
蛹虫草菌/虫草素/双重营养缺陷型突变Key words
Cordyceps militaris/cordycepin/double auxotrophic mutagenesis引用本文复制引用
王亮,刘文静,曹照平,田晶,金朝霞,周广麒..双重营养缺陷型蛹虫草突变株的选育及其高产虫草素的研究[J].现代食品科技,2017,33(3):54-59,6.基金项目
辽宁省教育厅科学研究一般项目(715020) （715020）
大连工业大学青年基金项目(67007907) （67007907）
