现代食品科技2017，Vol.33Issue(3)：74-80,7.DOI:10.13982/j.mfst.1673-9078.2017.3.012
从头合成赤松素大肠杆菌工程菌的构建
De Novo Biosynthesis of Pinosylvin Using Engineered Escherichia coil
摘要
Abstract
Pinosylvin,a stilbenoid analog of the valuable nutrient resveratrol,exhibits many biological effects,such as cardiovascular disease prevention and anticancer and anti-arthritic activities.Herein,genetic engineering procedures were used to develop a de novo method for pinosylvin synthesis in crder to ease the current problem of low pinosylvin supply.A constitutive vector containing the phenylalanine ammonia-lyase,cinnamoyl-coenzyme A ligase,and stilbene synthase genes was transformed into the L-phenylalanine-overproducing host swain Escherichia coli ATCC31884.PCR identification confirmed that a recombinant bacterial strain was obtained.The engineered bacterial cells were grown in shaking culture,and the culture medium was measured by HPLC to confirm that an engineered swain with de novo pinosylvin biosynthetic ability had been successfully created.Testing of culture samples taken at different fermentation times revealed that the rate of pinosylvin production increased more slowly after 12 h of culture,the highest pinosylvin yield of 0.32 mg/L was reached after a 24 h fermentation,and the maximum accumulated amount of cinnamic acid was 52.92 mg/L.These results suggest that the engineered E.coli can employ its own metabolism to synthesize pinosylvin de novo without addition of any other substances.However,the conversion efficiency of cinnamic acid was low,and thus further studies are needed to improve the strain.关键词
赤松素/大肠杆菌/从头合成Key words
pinosylvin/Escherichia coli/de novo biosynthesis引用本文复制引用
梁景龙,郭丽琼,林俊芳,蔡法及,陆雅琴..从头合成赤松素大肠杆菌工程菌的构建[J].现代食品科技,2017,33(3):74-80,7.基金项目
国家自然科学基金项目(31272217,31572178) （31272217,31572178）
广东省科技计划项目(2013B010404041,2014B050505018,2014B050505018) （2013B010404041,2014B050505018,2014B050505018）
