现代食品科技2017，Vol.33Issue(3)：191-195,178,6.DOI:10.13982/j.mfst.1673-9078.2017.3.029
环丙沙星衍生物的制备及其抗体特异性研究
Preparation of Ciprofloxacin Derivative and its Antibody Specificity
摘要
Abstract
The preparations of the ciprofloxacin (CPLX) carboxyl derivative and its antibody were studied in this paper,and the antibody specificity of this derivative was detemined.CPLX derivatives (CPLX-COOH) were synthesized with CPLX and 3-bromopropionic acid,and purified by preparation of liquid phase.Infrared spectroscopy (IR),electrospray ionization mass spectrometry (ESI-MS),and nuclear magnetic resonance (NMR) spectroscopy were used to identify the CPLX derivative.CPLX-COOH was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to prepare the immune antigen CPLX-COOH-BSA and coating antigen CPLX-COOH-OVA,which were identified using ultraviolet spectroscopy.The BALB/c mice were immunized with CPLX-COOH-BSA to obtain a polyclonal antibody directed against the CPLX derivative with high titer.The optimum concentration of the coating antigen and the optimum dilution of the antibody were determined using the chessboard method,and the indirect competitive enzyme-linked immunosorbent assay (ELISA) was established.The half-maximal inhibitory concentration (IC50) and the limit of detection (LOD) of CPLX-COOH were 228.56 ng/mL and 25.527 ng/mL,respectively.This antibody had a broad specificity and cross reactivity with multiple fluoroquinolones,and could be used to detect several fluoroquinolone residues.关键词
环丙沙星/间隔臂/衍生物/抗体/检测/ELISAKey words
ciprofloxacin/spacer arm/derivatives/antibody/detection/enzyme-linked immunosorbent assay引用本文复制引用
张明柱,冯亭亭,魏东..环丙沙星衍生物的制备及其抗体特异性研究[J].现代食品科技,2017,33(3):191-195,178,6.基金项目
河北省科技支撑计划项目(13227110D) （13227110D）
河北北方学院重大项目(ZD201403) （ZD201403）
河北北方学院创新人才基金项目(CXRC1303) （CXRC1303）
