现代食品科技2017，Vol.33Issue(3)：279-284,6.DOI:10.13982/j.mfst.1673-9078.2017.3.042
邻苯二甲酸二甲酯免疫层析快速检测试纸的研制
Development of a Colloidal Gold Immunochromatographic Strip for the Rapid Determination of Dimethyl Phthalate
摘要
Abstract
Phthalate ester plasticizer,a type of environmental hormone that is toxic to reproductive systems,has become a serious health threat because of its illegal transport and addition into foods intended for human consumption.Thus,development of an in situ testing method for the rapid detection of the plasticizer is essential.Herein,a colloidal gold-labeled immunochromatographic test paper method was developed for the rapid detection of dimethyl phthalate (DMP).To prepare the gold-labeled antibodies,anti-DMP polyclonal antibodies were labeled with colloidal gold prepared by the reduction of HAuCl4 using trisodium citrate.The chessboard method and the single-factor test were used to determine the optimal coating concentration and secondary antibody concentration,which were revealed to be 3000 μg/mL and 1600 μg/mL,respectively.The antibody was diluted 1.4-fold.The limit of detection of the developed assay was 0.4 μg/mL,and the visual detection time was within 5 min.Cross-reaction experiments showed no interference from substances with similar structures,indicating the high specificity of the method.The results from strip testing of baijiu liquor were consistent with those from GC-MS.This proposed immunochromatographic assay is fast,simple,and reliable,providing a valid method for the rapid in situ detection of DMP in foods.关键词
胶体金/层析/邻苯二甲酸甲酯/快速检测Key words
colloidal gold/chromatography/dimethyl phthalate/rapid detection引用本文复制引用
朱帆,邓丽华,朱彬,杨金易,徐振林,孙远明,王弘,沈玉栋..邻苯二甲酸二甲酯免疫层析快速检测试纸的研制[J].现代食品科技,2017,33(3):279-284,6.基金项目
广东省科技计划(2013B040402006,2014A020219008) （2013B040402006,2014A020219008）
国家自然科学基金项目(31371769) （31371769）
广州市科技计划(2011Y2-00007,2013J4100053) （2011Y2-00007,2013J4100053）
广东省优秀博士学位论文作者项目 （）
