现代食品科技2017，Vol.33Issue(3)：285-294,278,11.DOI:10.13982/j.mfst.1673-9078.2017.3.043
茯砖茶中黄酮类化合物的分离与鉴定
Isolation and Identification of the Flavones from Fuzhuan Tea
摘要
Abstract
A method for the preparative separation of flavone compounds from Fuzhuan tea using high-speed counter-current chromatography coupled with high-performance liquid chromatography (HSCCC-HPLC) is established in this study.The n-butanol-ethyl acetate-cetonitrile-0.5％ acetic acid (12∶2∶3∶15,V/V/) was used as the solvent system for HSCCC,and seven fractions were first separated by HSCCC.Subsequently,each fraction was isolated by the preparative HPLC and 19 compounds were obtained.Fifteen compounds were identified by the spectral methods and all of them were flavonoids:apigenin-bis-6,8-C-β-D-glucopyranoside (1),apigenin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (2),apigenin-7-O-β-D-galactopyranosyl-8-C-β-D-glucopyranoside (3),quercetin-3-O-β-D-glucopyranosyl-(1-3)-L-rhamnopyranosyl-(1-6)-β-D-glucopyranoside (4),kaempferol-3-O-β-D-glucopyranosyl-(1-3)-α-L-rhannopyranosyl-(1-6)-β-D-glucopyranoside (5),apigenin-7-O-α-L-rhamnopyranosyl-8-C-β-D-galactopyranoside (6),rutin (7),kaempferol-3-O-α-L-rhamnopyranosyl-(1-6)-β-D-glucopyranoside (8),camelliquercetiside A (9),myricetin-3-O-β-D-glucopyranoside (10),isovitexin (11),vitexin (12),quercetin-3-O-β-D-glucopyranoside (13),camelliquercetiside C (14),kaempferol-3-O-β-D-galactopyranoside (15).Among them,compound 3 and compound 6 were new C-glycosyl flavones and compound 1 was isolated from tea for the first time.关键词
茯砖茶/高速逆流色谱/制备液相/黄酮/分离Key words
Fuzhuan tea/high-speed countercurrent chromatography (HSCCC)/preparative high-performance liquid chromatography (HPLC)/flavones/isolation引用本文复制引用
陆英,钟晓红,操君喜,林海燕,陈栋,刘仲华..茯砖茶中黄酮类化合物的分离与鉴定[J].现代食品科技,2017,33(3):285-294,278,11.基金项目
国家茶叶产业技术体系建设专项CARS-23 （）
湖南省教育厅项目(15A088,15C0670) （15A088,15C0670）
