乳业科学与技术2017，Vol.40Issue(1)：16-18,3.DOI:10.15922/j.cnki.jdst.2017.01.004
高效液相色谱法测定乳制品中三氯蔗糖的含量
High Performance Liquid Chromatography Determination of Sucralose in Dairy Products
赵贞 1刘丽君 1李素琴 1岳虹 1李翠枝 1邵建波1
作者信息
- 1. 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特 010110
- 折叠
摘要
Abstract
A method for the determination of sucralose in dairy products by high performance liquid chromatography (HPLC) was developed.Samples were deproteinized by adding lead acetate followed by cleanup on an Oasis HLB solid-phase extraction cartridge.The separation was performed on a C18 column with 15％ acetonitrile in water as the mobile phase,and the analyte was detected with an evaporative light-scattering detector (ELSD) detector.The recovery of sucralose at spiked concentration levels of 7.5-70 mg/kg was 94.6％-101.3％.The limit of quantitation at a signal-to-noise ratio of 10 was 7.5 mg/kg.This method proved to be accurate,simple and suitable for the analysis of sucralose in dairy products.关键词
高效液相色谱/三氯蔗糖/乳制品/蒸发光散射检测器Key words
high performance liquid chromatography (HPLC)/sucralose/dairy products/evaporative light-scattering detector (ELSD)分类
轻工纺织引用本文复制引用
赵贞,刘丽君,李素琴,岳虹,李翠枝,邵建波..高效液相色谱法测定乳制品中三氯蔗糖的含量[J].乳业科学与技术,2017,40(1):16-18,3.
