食品与发酵工业2016，Vol.42Issue(5)：56-61,6.DOI:10.13995/j.cnki.11-1802/ts.201605010
烟碱对东方伊萨酵母HN-1乙醇发酵的影响
Effect of nicotine on ethanol fermentation of Issatchenkia orientalis HN-1
摘要
Abstract
The effects of nicotine on the ethanol fermentation by Issatchenkia orientalis HN-1 were studied using industrial strain Saccharomyces cerevisiae 1308 as control to provide valuable strain resources for the comprehensive utilization of tobacco stalk and other tobacco waste.The results indicated that nicotine could inhibit cell growth of these two strains significantly.After addition of nicotine from 0.1％ to 0.5％,ethanol production of Issatchenkia orientalis HN-1 and Saccharomyces cerevisiae 1308 was 18.17 ～9.51 g/L and 11.95 ～5.42 g/L,respectively,which was decreased by 20.81％ ～58.55％ and 40.42％ ～ 72.94％ compared with the blank without addition of nicotine.Meanwhile,the fermentation period of strain 1308 postponed 12 h compared with the blank control,and the inhibition of nicotine on glucose consumption in this group was also stronger than that treated with strain HN-1.Analysis of byproducts showed that the yield of acetic acid of strain HN-1 was 0.11 ～ 0.43 g/L,which was lower than that of strain 1308 with the yield of 0.20 ～0.59 g/L.However,glycerol production of strain HN-1 was 2.20 ～ 2.71 g/L,with was significantly higher than that of strain 1308 with the production of 1.48 ～2.33 g/L.Issatchenkia orientalis HN-1 was more suitable for bioconversion of tobacco stalk and other tobacco waste into ethanol compared with Saccharomyces cerevisiae 1308.关键词
酵母菌株/乙醇发酵/烟碱胁迫/耐受性Key words
yeast strains/ethanol fermentation/nicotine stress/tolerance引用本文复制引用
王风芹,付晨青,谢瑶嬛,谢慧,任天宝,宋安东..烟碱对东方伊萨酵母HN-1乙醇发酵的影响[J].食品与发酵工业,2016,42(5):56-61,6.基金项目
河南省高校科技创新团队支持计划(15IRTSTHN014) （15IRTSTHN014）
