食品与发酵工业2016，Vol.42Issue(5)：62-67,6.DOI:10.13995/j.cnki.11-1802/ts.201605011
高压脉冲电场对β-乳球蛋白结构和抗原性的影响
Effect of pulsed electric fields on structure and antigenicity of β-lactoglobulin
摘要
Abstract
The aim of this study was to research the effect of pulsed electric fields (PEF) on structure and allergenicity of ββ-lactoglobulin(β-Lg).SDS-PAGE,circular dichroism spectra,fluorescence spectra.and indirect competitive enzyme-linked immunosorbent assay were used to measure the structure and antigenicity,respectively.The results showed that PEF had no significant influence on the molecular weight of β-Lg.However,it had an significant effect on secondary structure,tertiary structure and antigenicity.Moreover,the change of antigenicity was closely related to that of the structure induced by PEF.The content of S-sheet,intrinsic fluorescence intensity,surface hydrophobicity and antigenicity were first increased and then decreased with the increasing of PEF treating time.The allergenicity of β-Lg reached up to 1344μg/mL when treated at 25 kV/cm for 30 μs.And it was decreased about 58％ at 210 μs.This indicated that the PEF could be used in producing low allergic milk.关键词
脉冲电场/β-乳球蛋白/抗原性/酶联免疫吸附法/结构Key words
pulsed electric fields/β-lactoglobulin/antigenicity/enzyme-linked immunosorbent assay/structure引用本文复制引用
涂宗财,李雪,王辉,杨文华,田明,马达..高压脉冲电场对β-乳球蛋白结构和抗原性的影响[J].食品与发酵工业,2016,42(5):62-67,6.基金项目
非热加工控制食物过敏原蛋白致敏性的脱敏制备关键技术(2013AA102205)国家863项目子课题 （2013AA102205）
