食品与发酵工业2016，Vol.42Issue(5)：102-107,6.DOI:10.13995/j.cnki.11-1802/ts.201605018
枯草芽孢杆菌产中性蛋白酶发酵条件优化
Optimization of fermentation conditions for the production of neutral protease by Bacillus subtilis
摘要
Abstract
The fermentation conditions for the production of neutral protease by Bacillus subtilis were investigated using single factor and orthogonal design experiments.The results showed that the optimal fermentation medium in the flasks contained 40 g/L glycerin,30 g/L soymeal,10 g/L bran,4 g/L Na2HPO4,and 0.3 g/L K2HPO4.The optimal fermentation conditions in the shaker were culturing with 6％ inoculum amount at 30 ℃ and 250 r/min.Under this condition,the enzyme activity reached 6 082.7U/mL.To further verify the optimized fermentation condition,the glycerol was fed and batch fermentation was carried out in 15 L fermenter.The enzyme activity reached 11 871 U/mL.关键词
中性蛋白酶/枯草芽孢杆菌/发酵优化Key words
neutral protease/Bacillus subtilis/fermentation optimization引用本文复制引用
李洪康,李由然,李赢,顾正华,丁重阳,石贵阳,张梁..枯草芽孢杆菌产中性蛋白酶发酵条件优化[J].食品与发酵工业,2016,42(5):102-107,6.基金项目
国家高技术研究发展计划(863计划)项目(2011AA100905) （863计划）
