食品与发酵工业2016，Vol.42Issue(5)：264-271,8.DOI:10.13995/j.cnki.11-1802/ts.201605046
芽胞杆菌基因敲除技术及其在工农业应用中的研究进展
Research progress on gene knock-out of Bacillus and its industrial and agricultural application
摘要
Abstract
The gene knock-out technology is a powerful tool for genetic engineering of Bacillus for the use in industry and agriculture.However,effective gene knock-out technology remains unavailable to many Bacillus strains with important application values.In recent years,researchers made noticeable progresses in developing widely applicable gene knock-out technology for Bacillus.Herein,on the basis of brief introduction of the principle of gene knockout,we mainly describe recent advances in the widely applicable gene knock-out methods for Bacillus and summarize their limitations to provide suggestions for selection of gene knock-out strategies in industrial and agricultural breeding of Bacillus.Finally,based on our previous research,we envision the development of the new gene knock-out technology for Bacillus in the future.关键词
芽胞杆菌/基因敲除/遗传育种/转化/同源重组Key words
Bacillus/gene knock-out/genetic breeding/transformation/homologous recombination引用本文复制引用
蒋宝莹,裘娟萍,孙东昌..芽胞杆菌基因敲除技术及其在工农业应用中的研究进展[J].食品与发酵工业,2016,42(5):264-271,8.基金项目
浙江省自然科学基金(No.LY16C010003) （No.LY16C010003）
杭州市科协人才专项资金(2012院士工作站建没项目)资助 （2012院士工作站建没项目）
