轻工学报2017，Vol.32Issue(2)：26-32,7.DOI:10.3969/j.issn.2096-1553.2017.2.005
固定化卤醇脱卤酶的制备及催化合成ATS-5的稳定性考察
Preparation of immobilized halohydrin dehalogenase and the stability testing of catalytic systhesis ATS-5
摘要
Abstract
The immobilization conditions of halohydrin dehalogenase (HHDH) by the sodium alginate were studied.The optimum conditions of immobilization process were as follows: sodium alginate concentration 3.5% , HHDH enzymatic amount 2.4×10 4 U/mL, glutaraldehyde concentration 1.00%, calcium chloride solution concentration 1.5% and immobilization time 2 h.Under the optimum immobilization conditions, the encapsulation efficiency of HHDH was 52.1%, the activity recovery of HHDH was 75.2%.After five batches of intermittent catalytic synthesis of (R)-4-cyano- 3-hydroxybutyric acid ethyl (ATS-5), the residual activity of immobilized HHDH was 51.2%. After the continuous catalytic synthesis of ATS-5 for 10 d,the substrate conversion efficiency was 90.6%, the distillation purification yield of ATS-5 was 98.2%, the purity and optical purity of ATS-5 were 99.3% and 99.1%, respectively .No obvious activity loss was obtained for immobilized HHDH after 80 d storage at 4 ℃.关键词
(R)-4-氰基-3-羟基丁酸乙酯/固定化/卤醇脱卤酶/催化稳定性Key words
(R)-4-cyano-3- hydroxybutyric acid ethyl/immobilization/ halohydrin dehaloge- nase/catalytic stability分类
生物科学引用本文复制引用
梁国斌,翟良创,汪斌,姚佳,周全法..固定化卤醇脱卤酶的制备及催化合成ATS-5的稳定性考察[J].轻工学报,2017,32(2):26-32,7.基金项目
江苏省产学研联合创新基金项目(BY2015028-04) （BY2015028-04）
