食品与发酵工业2017，Vol.43Issue(3)：42-47,6.DOI:10.13995/j.cnki.11-1802/ts.201703008
亚硝酸盐对植物乳杆菌FQR细胞表面性质及形态的影响
Effect of nitrite on the cell surface property and morphology of Lactobacillus plantarum FQR
摘要
Abstract
Effects of different concentrations of NaNO2 on the degradation ability,nitrite reductase (NiR) activity and cell surface properties of Lactobacillus plantarum (Lp) FQR were investigated,and the cell morphology of which was observed by electron microscope.The results showed that the degradation ability and NiR activity of FQR was decreasing with the increase of NaNO2 concentrations within 0-0.150％.Under the condition of 0.045％ NaN02,FQR could efficiently degrade NaNO2 within 16 h with (94.85 ± 2.96)％ of degradation rate and (174.60 ± 13.67) U/104 of NiR activity.In addition,high concentrations of NaN02 could also contribute to the significant increase of cell surface hydrophobicity and membrane permeability.There were no differences in cell surface morphology of FQR between the nitrite-free group and 0.045％ NaNO2 group,whereas the surface of FQR exposed to 0.120％ NaNO2 appeared depression and wrinkle by observation through scanning electron microscope.FQR in nitrite-free group exhibited numerous energy storage granules,number of which decreased obviously with increasing concentration of NaNO2.The intracellular energy storage material almost disappeared,and external flow of cytoplasm was observed through transmission electron microscope when FQR exposed to 0.120％ NaNO2.In summary,high concentrations of NaNO2 can cause changes in the surface morphology and internal structure of bacteria obviously.关键词
亚硝酸盐/植物乳杆菌/亚硝酸盐还原酶/细胞表面性质/电镜Key words
nitrite/Lactobacillus plantarum/nitrite reductase/cell surface property/electron microscope引用本文复制引用
韦田,梅林,王志耕,薛秀恒..亚硝酸盐对植物乳杆菌FQR细胞表面性质及形态的影响[J].食品与发酵工业,2017,43(3):42-47,6.基金项目
安徽省生猪产业技术体系建设专项资金(ahszcytx-5) （ahszcytx-5）
