食品与发酵工业2017,Vol.43Issue(3):54-60,7.DOI:10.13995/j.cnki.11-1802/ts.201703010
群体感应AHLs对温和气单胞菌体外致腐因子分泌的影响
Influence of quorum sensing AHLs on spoilage factor secretion of Aeromonas sobria
摘要
Abstract
Aeromonas sobria AS7 separated from spoilage turbot were used as test strain.The relationship between different treatment (the nutrition of substrate,symbiotic cultivation and adding different kind of AHLs) and the spoilage factors (siderophore,protase and biofilm) were further studied.The results showed that the secretion of siderophore was affected by medium quality,that could be secret at natural substrate such as skim milk,while could not be produced at synthetic medium.Addition of AHLs had a significant influence on the production of siderophore,wherein C8-HSL was affected significantly and the production of siderophore presented positive correlation with the increase AHLs (P < O.05),while effect on C6-HSL was not significant (P > 0.05).The production of siderophore of Pseudomonas fluorescens and Shewanella putrefaciens was obvious promoted when symbiotic cultivation with AS7.Adding AHLs could also promote the production of protase and biofilm,and present the positive correlation (P <0.05),which demonstrated that the AHLs could regulate the production of extracellular protease,siderophore and biofilm in AS7.关键词
温和气单胞菌/嗜铁素/胞外蛋白酶/生物被膜/群体感应Key words
Aeromonas sobria/siderophore/protase/biofilm/quorum sensing引用本文复制引用
李婷婷,崔方超,马艳,励建荣..群体感应AHLs对温和气单胞菌体外致腐因子分泌的影响[J].食品与发酵工业,2017,43(3):54-60,7.基金项目
国家自然科学基金(31301572,31471639) (31301572,31471639)
中国博士后科学基金(2014M552302) (2014M552302)