食品与发酵工业2017，Vol.43Issue(3)：224-228,5.DOI:10.13995/j.cnki.11-1802/ts.201703039
高效液相色谱法测定四角蛤蜊、菲律宾蛤仔中呈味核苷酸
Determination of flavor nucleotides in Mactra veneriformis and Ruditapes philippinarum by high performance liquid chromatography
摘要
Abstract
A method was established for determination of flavor nucleotides in Mactra veneriformis and Ruditapes philippinarum by high performance liquid chromatography.Methods:Separation and quantization were perfored by using a Waters Atlantis T3 column,with 0.01 mol/L KH2PO4-methanol as the mobile phase in gradient elution.The detection wavelength was 260 nm.Results:The external standard calibration curves were used for quantization.A good linear correlations for flavor nucleotide was shown with R2 ＞ 0.999 9.The average spike recovery of flavor nucleotides was 96.19％-103.25％,with relative standard deviations of 96.19％-103.25％.Conclusion:The method is simple,with good reproducibility,sensitivity and high accuracy.It is suitable for determining flavor nucleotides in Mactra veneriformis and Ruditapes philippinarum.The method provideds a basis for the research of shellfish flavor and the development of shellfish food.关键词
高效液相色谱法(high performance liquid chromatography,HPLC)/四角蛤蜊/菲律宾蛤仔/呈味核苷酸Key words
high performance liquid chromatography (HPLC)/Mactra veneriformis/Ruditapes philippinarum/flavor nucleotides引用本文复制引用
张倩,刘睿,王欣之,程建明,张文英,杜俊潮,吴皓..高效液相色谱法测定四角蛤蜊、菲律宾蛤仔中呈味核苷酸[J].食品与发酵工业,2017,43(3):224-228,5.基金项目
国家公益性海洋行业专项基金(201305007,201405017) （201305007,201405017）
国家高技术研究发展计划(863计划,2013AA093003) （863计划,2013AA093003）
江苏省高校优势学科建设工程项目(PAPD) （PAPD）
江苏省青蓝工程 （）
江苏省“333”工程 （）
