中国食物与营养2017，Vol.23Issue(3)：37-39,3.
保健食品中番茄红素测定方法的建立
Determination on Lycopene in Health Foods by HPLC
摘要
Abstract
High pertormance liquid chromatography (HPLC) method was developed for determination on lycopene in health foods.The samples were dissolved in gallic acid tert-butyl ester (TBG) dichloromethane solution and extracted by means of ultrasonic.The sample extract was cleaned up by polytetrafluoroethylene (PTFE) membrane.C18 Column (250 mm × 4.6 mam,5 μ.m) was as chromatographic separation column,and the mixed solution of acetonitrile and methanol was as mobile phase under elution.The detection on lycopene was carried out by HPLC.The external standard method was used for quantitative analysis.The calibration curves for lycopene were indicated in the range of 5.0 ～200 μg/mL,and the detection limit was 0.5 μg/mL.The recoveries of lycopene ranged from 81％ to 96％ with relative standard deviations 31％ ～59％.The method was simple and rapid to meet the requirement for determination on lycopene in health foods.关键词
番茄红素/保健品/没食子酸叔丁酯/高效液相色谱Key words
lycopene/health food/gallic acid tert-butyl ester/high performance liquid chromatography (HPLC)引用本文复制引用
刘婷婷,程家丽,张雪松,沈葹,卓勤..保健食品中番茄红素测定方法的建立[J].中国食物与营养,2017,23(3):37-39,3.基金项目
十二五科技支撑项目“功能食品资源优化及评价共性关键技术研究”(项目编号:2012BAD33B01). （项目编号:2012BAD33B01）
