食品与机械2017，Vol.33Issue(3)：31-36,6.DOI:10.13652/j.issn.1003-5788.2017.03.007
牛类芽孢杆菌BD3526产抗菌物质培养条件的优化
Optimization on cultivation conditions for antimicrobial substance production by Paenibacillus bovis sp.nov.BD3526
摘要
Abstract
In order to increase the production of the antimicrobial substances by P.bovis BD3526 in wheat bran broth,the effect of individual fermentation parameter including cultivation temperature,period,inoculum ratio,age of the inoculum and concentration of wheat bran on the inhibitory intensity of the fermented wheat bran broth was determined,employing Micrococcus luteus as the indicator.Based on the results of single factor tests,four variables i.e.cultivation temperature,cultivation period,inoculum ratio and concentration of wheat bran were employed to further optimize the fermentation conditions for antimicrobial components production by the strain BD3526 by response surface methodology.The optimal cultivation conditions for BD3526 to produce antimicrobial substance were composed of a cultivation temperature of 29 ℃,a cultivation period of 73 h,an inoculum ratio of 3.2％ and a wheat bran concentration of 4.2％.The inhibitory zone of the cultivated wheat bran broth under the optimal condition on M.luteus reached 19.88 mm in diameter,and the potency of bacteriocin produced under the optimal conditions was raised by 100％ and 300％ as compared to that produced under the original fermentation conditions.关键词
牛类芽孢杆菌BD3526/抗菌物质/培养条件Key words
P.bovis BD3526/antimicrobial substance/cultivation conditions引用本文复制引用
花榜清,张秋香,刘振民,吴正钧..牛类芽孢杆菌BD3526产抗菌物质培养条件的优化[J].食品与机械,2017,33(3):31-36,6.基金项目
国家“十二五”科技支撑计划(编号:2013BAD18B01) （编号:2013BAD18B01）
上海市科委项目(编号:16DZ2280600) （编号:16DZ2280600）
