食品与机械2017，Vol.33Issue(3)：139-144,6.DOI:10.13652/j.issn.1003-5788.2017.03.029
玉米黄色素提取工艺优化及其抗癌活性研究
Extraction process optimization and anticancer activity of maize yellow pigment
摘要
Abstract
Objective:To study the best preparation technology of maize yellow pigment by ultrasonic assisted with enzymatic method,and the inhibitory effect of them on the growth of human gastric cancer cells (BGC-823).Methods:The optimum conditions of maize yellow pigment were extracted by ultrasonic assisted with enzymatic method by single factor and response surface experiments.The inhibition of maize yellow pigment on BGC-823 cells was detected by morphological observation and MTT assay to evaluate the antitumor activity.Results:the maize yellow pigment best extraction condition of ultrasonic with enzymatic method is:enzyme activity 10 000 U/g,ultrasonic power 400 W,enzymolysis temperature 35 ℃,the maximum extraction yield was (72.64 ± 0.42) μg/g under the condition,the maize yellow pigment has a certain inhibitory effect on BGC-823cell were studied by cell morphological observation and MTT test.Conclusion:The extraction of maize yellow pigment by ultrasonic assisted with enzymatic method is a kind of effective extraction method,the maize yellow pigment prepared has strong inhibitory effect on BGC-823 cells.关键词
玉米黄色素/超声波/酶法/抗癌Key words
maize yellow pigment/ultrasonic/enzymic method/anticancer引用本文复制引用
于慧,刘美佳,刘剑利,曹向宇..玉米黄色素提取工艺优化及其抗癌活性研究[J].食品与机械,2017,33(3):139-144,6.基金项目
国家自然科学基金委员会科学部主任基金项目(编号:31240005) （编号:31240005）
辽宁省农业领域青年科技创新人才培养资助计划项目(编号:2015013) （编号:2015013）
辽宁省高等学校优秀人才支持计划项目(编号:LJQ2013002) （编号:LJQ2013002）
辽宁省高等学校科学研究一般项目(编号:L2014007) （编号:L2014007）
辽宁大学博士科研启动项目 （）
