食品与机械2017，Vol.33Issue(3)：145-149,206,6.DOI:10.13652/j.issn.1003-5788.2017.03.030
山皂荚多糖的提取工艺及抑菌活性
Extraction of polysaccharide from Gleditsia japonic.miq and its antibacterial activities
摘要
Abstract
The polysaccharide in Gleditsia japonic.miq was researched.Based on the single factor level determination,the major factors including liquid/material ratio,extraction temperature and time were investigated by response surface analysis,and then its antibacterial activity was studied by filter paper diffusion method.The results showed that the optimum technology condition were using liquid/material at 50 mL/g,extracted at 65 ℃ for 80 min.Under these conditions,the yield of polysaccharide was (31.52±0.54) ％.Furthermore,when the concentration of polysaccharide was 100 mg/mL,theantibacterial activity was in this order,S.typhimurium ＜Escherichia coli ＜ Bacillus cereus ＜ Bacillus subtilis ＜Staphylococcus aureus,and the minimal inhibitory concentrations of polysaccharide against Staphylococcus aureus,Escherichia coli,S.typhimurium,Bacillus subtilis,and Bacillus cereu were 25,50,50,25,50 mg/mL,respectively.关键词
山皂荚/多糖/抑菌活性Key words
Gleditsia japonic.miq/polysaccharide/antibacterial activities引用本文复制引用
高洁,董文宾,王勇,王燕..山皂荚多糖的提取工艺及抑菌活性[J].食品与机械,2017,33(3):145-149,206,6.基金项目
陕西省教育厅科研计划项目(编号:12JK1044) （编号:12JK1044）
陕西省咸阳市科技局科学技术研究项目(编号:2015K04-20) （编号:2015K04-20）
