食品与机械2017，Vol.33Issue(3)：168-173,6.DOI:10.13652/j.issn.1003-5788.2017.03.035
双水相萃取分离葡萄籽中原花青素
Separation of procyanidin from grape seed by aqueous two-phase system
摘要
Abstract
Procyanidin from grape seed were separated and purified by the aqueous phase two-extraction.Aqueous two-phase extraction systems,differently assisted extraction methods and the extraction strategies were firstly studied.The best extractions of procyanidin by aqueous two-phase system were investigated based on orthogonal experimental design.The results showed that,in the aqueous twophase extraction system of ethanol and (NH4)2 SO4,the extraction method was the microwave-assisted extraction,i.e.,Ethanol,water,and compounded by microwave treatment,as well as (NH4)2 SO4 were added.It was found that the best extraction conditions were 30％(w/w) ethanol/20％(w/w) (NH4)2SO4,3％(w/w) grape seed powders,pH=4.0,microwave power 100W,,and microwave time 5 min.Under these best extraction conditions,the recovery rate of procyanidin was 94.1％,and its yield was 25.8 mg/g.关键词
葡萄籽/原花青素/双水相萃取Key words
grape seed/procyanidin/aqueous two-phase extraction引用本文复制引用
张喜峰,程广,何倩,张芬琴,罗光宏..双水相萃取分离葡萄籽中原花青素[J].食品与机械,2017,33(3):168-173,6.基金项目
甘肃省中小企业创新基金项目(编号:1047GCCG001) （编号:1047GCCG001）
国家级大学生创新创业训练计划项目(编号:201510740005) （编号:201510740005）
