沈阳农业大学学报2017，Vol.48Issue(2)：166-173,8.DOI:10.3969/j.issn.1000-1700.2017.02.006
两株鹿源芽孢杆菌的分离鉴定及生物学特性研究
Isolation, Identification and Biological Characteristics of Two Strains of Deer-derived Bacillus
摘要
Abstract
In order to obtain the Bacillus in the intestinal flora of deer and study its beneficial effects, intestinal secretion of Sika Deer was processed with 85℃ water-curing treatment for 15 min, and Bacillus were screened. Bacillus was identified according to the colony morphology, physiological and biochemical characteristics and molecular biological results. The biological characteristics were studied by the methods of antibacterial test, acid resistance, salt tolerance test and cellulase production test. Results showed that four strains of Bacillus were isolated from the experiment, named XF-1, XF-2, XF-3 and XF-4. Two of them were identified, XF-1 was Bacillus licheniformis and XF-3 was Bacillus amyloliquefaciens. The inhibitory effect of two strains of Bacillus on the four pathogenic bacteria of was studied by using the Oxford cup method. XF-1 had a good inhibitory effect on Escherichia coli, Streptococcus and Salmonella; XF-3 had a good inhibitory effect on Escherichia coli, Staphylococcus aureus, Streptococcus, Salmonella. CMC-Na medium was used to detect the cellulase producing ability of two strains of Bacillus. The cellulose degradation index of strain XF-1 was D/d=2.18, the cellulose degradation index of strain XF-3 was D/d=3.18, and the cellulose degrading capacity of XF-3 was higher than XF-1.In the experiment, the survival rate of bacteria in different acid environment and bile salt environment was calculated, and the difference of tolerance was evaluated. The results were indicated that after 24h,the survival rate of XF-1 in the pH 3.0 environment was 7.3%, the survival rate was 8.57% in the 0.2% bile salt environment. Cellulase was produced after 24h, the survival rate of XF-3 in the pH 3.0 environment was 12.5%, the survival rate was 6.67% in the 0.2% bile salt environment. The results showed that the two strains had a certain degree of resistance and probiotic ability. They had been used for research and development as probiotics.关键词
芽孢杆菌/鹿源/分离鉴定/生物学特性Key words
Bacillus/deer-derived/isolation and identification/biological characteristics分类
农业科技引用本文复制引用
张彤,何剑斌,李鹏,龙淼,张燚,郑伟萍,姜丽莹,杨淑华..两株鹿源芽孢杆菌的分离鉴定及生物学特性研究[J].沈阳农业大学学报,2017,48(2):166-173,8.基金项目
辽宁省科技厅项目(201501,201601) （201501,201601）
