沈阳农业大学学报2017，Vol.48Issue(2)：232-237,6.DOI:10.3969/j.issn.1000-1700.2017.02.015
N-乙酰半胱氨酸对脓毒症大鼠肠损伤的保护作用及对炎症因子表达的影响
Protective Effects of N-on Intestinal Injury Induced by Sepsis in Rats and Its Effect on the Expression of Inflammatory Factors
摘要
Abstract
To investigate the effect of N-acetylcysteine (NAC) on intestinal injury in rats with sepsis and its effect on the expression of inflammatory factors, 30 SD rats of clean grade, weighing 250 to 300 g, were randomly divided into sham operation group (Sham group), sepsis group (SEP group) and NAC treatment group (NAC group), 10 rats in each group. Group Sham was only exposed to laparotomy, cecum, NAC group and SEP group underwent cecal ligation and puncture operation, then to NAC 150 mg·kg-1 and intravenous saline. Three groups were in 4H reperfusion rats were sacrificed to observe the intestinal pathological detection. ELISA blood intestinal fatty acid binding protein (I-FABP) and D-lactate concentration; serum and intestine was detected by ELISA and Western blot in TNF-, IL-6, alpha the concentration of -10 IL. The results showed that under light microscope group Sham had no pathological injury and SEP injury group, NAC injury group reduced compared with S group, SEP group, I-FABP D-elevated lactate (p<0.05). Compared with SEP group, NAC group I-FABP, D-lactic acid decreased (p<0.05). Compared with Sham group, SEP group serum TNF-, IL-6, IL alpha-10 increased (p<0.05). Compared with SEP group, NAC group decreased TNF- alpha and IL-6 (p<0.05), IL -10 increased (p<0.05), the trend of changes of intestinal inflammation related factors with serum. N- acetylcysteine has protective effect on intestinal mucosal barrier in rats with sepsis, and its mechanism is related to inhibiting inflammatory reaction.关键词
N-乙酰半胱氨酸/脓毒症/大鼠/肠粘膜Key words
N-acetyl cysteine/sepsis/rat/intestinal mucosa分类
医药卫生引用本文复制引用
杨晨,陈雪婷,王猛,王俊平..N-乙酰半胱氨酸对脓毒症大鼠肠损伤的保护作用及对炎症因子表达的影响[J].沈阳农业大学学报,2017,48(2):232-237,6.基金项目
国家自然科学基金项目(81473625) （81473625）
