轻工学报2017，Vol.32Issue(3)：51-57,7.DOI:10.3969/j.issn.2096-1553.2017.3.009
烤烟品种的多重PCR技术标记鉴别
Tag identification of flue-cured tobacco varieties by multiplex PCR technique
摘要
Abstract
With 12 varieties of flue-cured tobacco as a template,the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized.Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR.The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR.Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately.关键词
多重PCR技术/SCAR标记/烤烟品种鉴别/指纹图谱Key words
multiplex PCR technique/SCAR tag/identifica-tion of flue-cured tobacco varieties/fingerprints map分类
轻工纺织引用本文复制引用
贾梦珠,马林,孙九喆,苏东赢,杨金初,王二彬,许衡,王永超,王君婷,孟丹丹..烤烟品种的多重PCR技术标记鉴别[J].轻工学报,2017,32(3):51-57,7.基金项目
河南中烟有限责任公司科技项目(ZW2015012) （ZW2015012）
