食品科学2017，Vol.38Issue(7)：23-28,6.DOI:10.7506/spkx1002-6630-201707005
葵花籽壳木质素的结构分析及抗氧化活性
Structural Features and Antioxidant Activities of Lignin Extracted from Sunflower Seed Shell
摘要
Abstract
Lignin was extracted from sunflower seed shell using acetic acid.The structural features of the isolated lignin were characterized by gel permeation chromatography (GPC),ultraviolet spectroscopy (UV),infrared spectroscopy (FT-IR),hydrogen-1 nuclear magnetic resonance spectrocopy (1H-NMR) and thermogravimetry (TG).In addition,the antioxidant activity of the lignin was evaluated by 1,1 diphenyl-2-trinitrobenzene hydrazine (DPPH) radical scavenging activity assay.The results showed that the yield and purity of lignin extracted from sunflower seed shell were 70.12％ (based on Klason lignin) and 88.54％,respectively,and the content of lignin-associated carbohydrates was 2.92％.The lignin was GS-type lignin with β-O-4 ether bond as the major inter-unit linkage,having a high proportion of guaiacyl (G) units.The weight average molecular (Mw),number-average molecular (Mn) and polydispersity (Mw/Mn) of the lignin were 2 977,1 931 D and 1.54,respectively.It major decomposition temperature was 200-400 ℃ and the maximum weight loss rate was 0.32％/℃.We found that the lignin showed stronger antioxidant activity than butylated hydroxytoluene (BHT) with free radical scavenging index (RSI) of 1.54 versus 0.94.The results suggested that acetic acid extraction provides a promising way to prepare lignin from sunflower seed shell with a good potential as an antioxidant in food and other industries.关键词
葵花籽壳/醋酸法/木质素/结构分析/抗氧化活性Key words
sunflower seed shell/acetic acid extraction/lignin/structural feature/antioxidant activity分类
轻工纺织引用本文复制引用
龚卫华,马玥,吕霞,叶发银,赵国华..葵花籽壳木质素的结构分析及抗氧化活性[J].食品科学,2017,38(7):23-28,6.基金项目
国家自然科学基金面上项目(31371737) （31371737）
重庆市特色食品工程技术研究中心能力提升项目(cstc2014pt-gc8001) （cstc2014pt-gc8001）
