食品科学2017，Vol.38Issue(7)：155-160,6.DOI:10.7506/spkx1002-6630-201707025
铁皮石斛多糖化学修饰及其对免疫活性的影响
Chemical Modification and Immunoregulatory Activity of Polysaccharides from Dendrobium officinale
摘要
Abstract
Highly pure polysaccharides from the dried stems of Dendrobium officinale were obtained by water extraction and alcohol precipitation combined with freeze-thawing,and they were further subjected to chemical modification by sulfation,deacetylation and carboxymethylation,respectively,with the aim of investigating the effects of these different modifications on the immunomodulatory activity ofDendrobium officinale polysaccharides in RAW264.7 macrophages.The results showed that Dendrobium officinale polysaccharides had high neutral sugar contents along with low contents ofuronic acids and proteins,which were 87.37％,3.19％ and 0.51％,respectively.The neutral sugar content ofDendrobium officinale polysaccharides was significantly reduced by sulfation and carboxymethylation.The Fourier transform infrared spectroscopic (FTIR) analysis indicated that sulfation,deacetylation and carboxymethylation derivatives were successfully prepared.The molecular weight ofDendrobium officinale polysaccharides measured by high-performance gel permeation chromatography (GPGPC) was 960 kD,while it was significantly increased by sulfation and earboxymethylation.It was found that sulfation and deacetylation could enhance the immunomodulatory activity of Dendrobium oficinale polysaccharides in vitro,while carboxymethylation had a significant opposite effect,indicating that grafted modification cannot necessarily improve the immunomodulatory activity of Dendrobium officinale polysaccharides.关键词
铁皮石斛/多糖/化学修饰/RAW264.7巨噬细胞/免疫活性Key words
Dendrobium officinale/polysaccharide/chemical modification/RAW264.7 macrophages/immunomodulatory activity分类
轻工纺织引用本文复制引用
童微,余强,李虎,崔武卫,聂少平..铁皮石斛多糖化学修饰及其对免疫活性的影响[J].食品科学,2017,38(7):155-160,6.基金项目
江西省主要学科学术和技术带头人培养计划项目(20162BCB22008) （20162BCB22008）
