食品科学2017，Vol.38Issue(7)：187-192,6.DOI:10.7506/spkx1002-6630-201707030
茶多糖对小鼠肠道健康及免疫调节功能的影响
Effects of Green Tea Polysaccharides on Intestinal Health and Immune Regulation in Mice
摘要
Abstract
The objective of this work was to evaluate the effect of tea polysaccharides (TPS,from Wuyuan green tea) on intestinal health and immune regulation in mice.Healthy C57/BL6 mice were randomly divided into four groups,and they were administrated by gavage with different concentrations of tea polysaccharides.After adaptive feeding for one week,the animals in these groups were given high,mediumand low doses of TPS and normal saline as blank control for 21 days,respectively.At the end of the experimental period,the mice were sacrificed,and spleen and thymus were harvested and weighted to calculate immune organ indexes.Peritoneal macrophages were used to examine phagocytosis activity by using neutral red phagocytosis assay.Short chain fatty acids (SCFAs) in colonic contents were analyzed by gas chromatography (GC) and ammonia by the indophenol blue colorimetric method.Meanwhile,the pH and water content of colonic contents were also measured.Results showed that immune organ indexes were increased significantly after TPS administration.The phagocytosis of peritoneal macrophages was improved in a dose-dependent manner.Some SCFAs (acetic acid,propionic acid and butyric acid) were increased while no difference in isobutyrate,pentanoic acid and delphinic acid was detected.A significant decline in the ammonia content and pH of colonic contents of mice was observed,while water content increased.Therefore,TPS can regulate immune function and promote intestinal health in mice.关键词
茶多糖/免疫调节/肠道健康Key words
tea polysaccharides/immune regulation/intestinal health分类
轻工纺织引用本文复制引用
李海珊,刘丽乔,聂少平..茶多糖对小鼠肠道健康及免疫调节功能的影响[J].食品科学,2017,38(7):187-192,6.基金项目
国家自然科学基金优秀青年科学基金项目(31422042) （31422042）
