食品科学2017，Vol.38Issue(7)：193-200,8.DOI:10.7506/spkx1002-6630-201707031
冬凌草甲素诱导小鼠肝癌细胞醌还原酶活性及其机理研究
Oridonin-Induced Quinone Reductase Activity and Its Mechanism in Mouse Hepatoma Hepa1c1c7 Cells
摘要
Abstract
To screen and identify the anticancer components of Rabdosia serra (Maxim) Hara,the effects of the crude ethyl acetate extract of Rabdosia serra (Maxim) Hara and its purified fractions obtained by silica gel column chromatography and semi-preparative chromatography on cell viability and quinone reductase (QR/NQO1) activity were evaluated using mouse hepatoma Hepa1c1c7 cells.The purified fractions were identified by mass spectrometry (MS),1H-nuclear magnetic renonsance (1H-NMR) and 13C-NMR.Furthermore,mouse hepatoma cells were pretreated with specific protein kinase inhibitors including phosphatidylinositol 3-kinase (PI3K) inhibitor,extracellular signal-regulated kinase (ERK) inhibitor,mitogen activated protein kinases(MAPK) p38 inhibitor,protein kinase C (PKC) inhibitor and c-Jun N-terminal kinase (JNK) inhibitor to determine the signal transduction pathways leading to the induction of QR activity.The mRNA and protein expression levels of NQO 1 were measured by quantitative PCR and Westem blot,respectively.Our results suggested as follows:1) oridonin was identified as a potential anticancer component of this extract;2) oridonin at a concentration of 0.94 μg/mL exhibited a remarkable potency in inducing QR;3) PI3K inhibitor significantly inhibited the induction of QR activity by oridonin,while ERK inhibitor showed an opposite effect.Moreover,p38,PKC and JNK inhibitors did not significantly affect the induction effect of oridonin on QR;4) oridonin markedly increased the expression of both mRNA and protein of NQO1.In conclusion,oridonin from Rabdosia serra (Maxim) Hara can induce QR activity in cultured Hepa1c1c7 cells by activating the PI3K signal transduction pathway and inhibiting the ERK signal transduction pathway,and it also can significantly induce the transcription and translation of NQO1.These results would provid a preliminary theoretical basis for cancer chemoprevention with Rabdosia serra (Maxim) Hara and oridonin.关键词
溪黄草/冬凌草甲素/醌还原酶/Hepa1c1c7细胞株/信号通路Key words
Rabdosia serra (Maxim) Hara/oridonin/quinone reductase/Hepa1c1c7 cell line/signal transduction pathway分类
生物科学引用本文复制引用
王佐,李斌,王璐,陈忠正,劳扬,刘杏宜,高雄,燕妮,林晓蓉,张媛媛..冬凌草甲素诱导小鼠肝癌细胞醌还原酶活性及其机理研究[J].食品科学,2017,38(7):193-200,8.基金项目
国家现代农业产业技术体系建设专项(CARS-23) （CARS-23）
