食品科学2017，Vol.38Issue(7)：213-218,6.DOI:10.7506/spkx1002-6630-201707034
低聚葡萄籽原花青素联合顺铂对A549细胞增殖及细胞周期的影响
Effect of Oligomeric Proanthocyanidins from Grape Seeds Combined with Cisplatin on A549 Cell Proliferation and Cell Cycle
摘要
Abstract
Objective:To investigate the effect of oligomeric proanthocyanidins from grape seeds (O-GSP) combined with cisplatin (DDP) on the proliferation,cell cycle and cell cycle-related protein expression of A549 cells.Methods:A549 cells were cultured in vitro.The effects of DDP and O-GSP alone and in combination on the survival rate of A549 cells were observed by MTT assay;the combined effect of O-GSP and DDP on the cell cycle of A549 cells was detected by flow cytometry and the expression levels of the cell cycle-related proteins CDK 4,Cyclin D1 and p-Rb were detected by Western blot.Results:DDP (≥ 5 mg/L) and O-GSP (≥ 16 mg/L) treatments alone could inhibit A549 cell proliferation.The combined treatment had a synergistic effect on the inhibition of cell proliferation.DDP treatment alone at 5 mg/L arrested A549 cells at S phase and O-GSP at 4 mg/L arrested the cells at G0/G1 phase.The combination of DDP at 5 mg/L and O-GSP at 4 mg/L significantly reduced the expression levels of the cell cycle regulatory proteins CDK 4,Cyclin D1 and p-Rb.Conclusion:DDP can inhibit the proliferation of A549 cells in combination with O-GSP through a mechanism related to the regulation of cell cycle progression mediated by the Cyclin D 1-CDK 4-Rb pathway.关键词
低聚葡萄籽原花青素/顺铂/细胞增殖/细胞周期Key words
oligomeric proanthocyanidins from grape seeds/cisplatin/cell proliferation/cell cycle分类
农业科技引用本文复制引用
王海,连燕娜,高丽萍..低聚葡萄籽原花青素联合顺铂对A549细胞增殖及细胞周期的影响[J].食品科学,2017,38(7):213-218,6.基金项目
北京市自然科学基金资助项目(7163211) （7163211）
