食品科学2017，Vol.38Issue(8)：6-10,5.DOI:10.7506/spkx1002-6630-201708002
产细菌素屎肠球菌TRS5特性分析及其编码基因的扩增
Characterization of Enterococcus faecalis TRS5, a Strain Able to Produce Bacteriocin and PCR Amplification of Its Bacteriocin-Encoding Genes
摘要
Abstract
The production of bacteriocin by Enterococcusfaecium TRS5 was found to be maximum at 37 ℃ and pH 6.5 after 24 h of incubation in MRS broth.The presence of tryptone or glucose in the medium stimulated the bacteriocin production.Added maltose,lactose or mannose (20 g/L) resulted in a 50％ reduction of bacteriocin activity.High levels of glycerol and Tween-80 (5 g/L) also repressed the bacteriocin production.The presence of K2HPO4 or vitamins VB1,VB2,VB6,or VC in the medium had no effect on the bacteriocin production.E.faecalis TRS5 was sensitive to erythromycin,chloramphenicol,vancomycin,teicoplanin,tetracycline and penicillin G.PCR amplification and sequencing demonstrated that E.faecalis TRS5 harbored enterocin P and L50-1ike structural genes.关键词
细菌素/屎肠球菌/细菌素的生成/扩增Key words
bacteriocin/Enterococcus faecalis/bacteriocin production/amplification分类
轻工纺织引用本文复制引用
荣梓娴,王蓉蓉,肖茜,刘成国,周辉..产细菌素屎肠球菌TRS5特性分析及其编码基因的扩增[J].食品科学,2017,38(8):6-10,5.基金项目
国家自然科学基金面上项目(31571811) （31571811）
湖南省教育厅科学研究项目(16K043) （16K043）
