食品科学2017，Vol.38Issue(8)：11-16,6.DOI:10.7506/spkx1002-6630-201708003
耐盐酵母尿嘧啶营养缺陷型菌株的构建
Construction of Uracil Auxotrophic Strains of Salt Tolerant Yeasts
摘要
Abstract
Candida versatilis (called T yeast) and Zygosaccharomyces rouxii (called S yeast) are often used as starter cultures to enhance the flavor quality of high-salt liquid-state soy sauce.However,there are currently no auxotrophic strains available for the genetic manipulation of S yeast and T yeast.Therefore,ethylmethane sulfonate (EMS) and ultraviolet (UV) irradiation were used to mutagenize the yeasts obtain uracil auxotrophic strains.On the basis of mortality rate,the optimal concentration of EMS,EMS treatment time,UV irradiation distance,UV irradiation time,and yeast concentration were determined.In the end,the uracil auxotrophic strains were screened out and identified by gene sequencing,which will lay the foundation for molecular biology studies of salt tolerant yeast.关键词
S酵母/T酵母/EMS诱变/紫外诱变/尿嘧啶营养缺陷型Key words
Candida versatilis/Zygosaccharomyces rouxii/ethylmethane sulfonate (EMS)/ultraviolet mutagenesis/uracil auxotrophic分类
轻工纺织引用本文复制引用
赵秀丽,张珊,宋璐,王檬,侯丽华..耐盐酵母尿嘧啶营养缺陷型菌株的构建[J].食品科学,2017,38(8):11-16,6.基金项目
国家自然科学基金面上项目(31371819) （31371819）
天津市科技特派员项目(15JCTPJC62500) （15JCTPJC62500）
