食品科学2017，Vol.38Issue(8)：49-55,7.DOI:10.7506/spkx1002-6630-201708009
不同茶类发花茯茶中“金花”菌的分离、鉴定及产黄曲霉毒素分析
Identification and Aflatoxin Production of ‘Golden Flora’ Fungi Isolated from Fu Tea Produced from Different Kinds of Tea
摘要
Abstract
Five different kinds of Fu tea were produced from oolong tea (Dahongpao),black tea,green tea,and tea leaves used for making two kinds of dark tea,Tianjian tea and Jinxiangyi brick tea,by fungal fermentation in a loose state.‘Golden Floral’ fungi isolated from these Fu teas showed morphological differences.Further differences were investigated by microbiological methods.The isolated fungi were systematically identified by morphology and DNA sequence analysis.Meanwhile,the aflatoxin producing ability of these isolates was determined by high performance liquid chromatography (HPLC)-triple quadrupole tandem mass spectrometry.The results revealed that all the ‘Golden Floral’ fungi isolated were assigned to the same species and they were not different from the initial inoculum,which were classified as Eurotium cristatum in the Eumycota genus in the Euroaceae family in the Eurotiales order in the Euascomycetes subclass in the Ascomycetes class in the Eumycota phylum and its anamorph was identified as Aspergillus spiculosus Blaser.And the mass spectral data showed that AFB1,AFB2,AFG1 and AFG2 were not detected,indicating that the ‘Golden Floral’ fungi are not able to produce aflatoxins.关键词
茯茶/散茶发花/“金花”菌/鉴定/冠突散囊菌/黄曲霉毒素Key words
Fu tea/fungal fermentation of tea in a loose state/‘Golden Floral’ fungi/identification/Eurotium cristatum/aflatoxin分类
农业科技引用本文复制引用
黄浩,郑红发,赵熙,银霞,黄怀生,粟本文,钟兴刚,黄建安,刘仲华..不同茶类发花茯茶中“金花”菌的分离、鉴定及产黄曲霉毒素分析[J].食品科学,2017,38(8):49-55,7.基金项目
湖南省自然科学基金(湖南省科学技术厅与湖南省农业科学院联合基金)项目(2016JJ6058) （湖南省科学技术厅与湖南省农业科学院联合基金）
国家自然科学基金面上项目(31471706) （31471706）
国家现代农业(茶叶)产业技术体系建设专项(CARS-23) （茶叶）
湖南省茶叶研究所所长基金项目(2015SJ01) （2015SJ01）
