食品科学2017，Vol.38Issue(8)：74-79,6.DOI:10.7506/spkx1002-6630-201708013
南海海域动物肠道中抗菌活性乳酸菌的分离及多样性分析
Separation and Diversity of Lactic Acid Bacteria Possessing Antimicrobial Activity Isolated from the Intestine of Marine Animals in South China Sea
摘要
Abstract
Lactic acid bacteria (LAB) were isolated from the intestine of 10 common kinds of marine animals collected from the South China Sea by the pour plate method.The antimicrobial activity of the LAB isolates was determined by Oxford cup diffusion method against five pathogenic indicator strains including E.coli,V.parahaemolytics,S.aureus,B.subtilis,and L.monocytogenes.The diversity analysis of these LAB strains was conducted by establishing phylogenetic tree based on 16S rRNA gene sequences.The results showed that 43 isolates,which presented soluble calcium circle and were positive by Gram staining and negative in catalase reaction,were identified as LAB according to morphological observation and partial physiological and biochemical experiments,and they all possessed antimicrobial activity in different degrees.Out of these,13 strains were found to have stronger bacteriostatic ability and a wider antimicrobial spectrum,and they were further identified based on phyogenetical analysis of 16S rRNA sequence.The results indicated that they belonged to 6 genera in 4 families of Firmicutes.Although strain LY-87 and the type strain of Lactococcus lactis subsp.lactis shared 100％ 16S rRNA gene sequence similarity,there were genetic differences between other LAB strains and the typical strains showing the closest phylogenetic relationship with them (similarity ranging from 95.7％ to 99.9％).The similarity between strain PQ-26 and the type strain of Pediococcus pentosaceus was only 95.7％ suggesting that it is likely to be a potential new species of Pediococcus.This research indicates that LAB with antimicrobial activity are diverse and there may exist potential new species in the intestine of animals from the South China Sea in Zhanjiang.The antimicrobial substances produced by these bacteria need to be further studied and developed.关键词
海洋动物肠道/抗菌活性/乳酸菌/16S rRNA基因/多样性Key words
marine animal intestine/antimicrobial activity/lactic acid bacteria/16S rRNA gene/diversity分类
生物科学引用本文复制引用
焦冬冬,任文彬,赵鸭美,侯小琴,刘颖,孙力军,王雅玲..南海海域动物肠道中抗菌活性乳酸菌的分离及多样性分析[J].食品科学,2017,38(8):74-79,6.基金项目
广东省科技计划项目(2014A020217018 （）
2016A020222014) （）
广东省水产品加工与安全重点实验室开放基金课题(GDPKLAPPS1503) （GDPKLAPPS1503）
国家自然科学基金面上项目(31371777 （）
31171634) （）
