食品科学2017，Vol.38Issue(8)：239-243,5.DOI:10.7506/spkx1002-6630-201708037
酶联免疫吸附法检测动物源性食品中恩诺沙星残留量
ELISA Determination of Enrofloxacin
摘要
Abstract
An indirect competitive enzyme-linked immunosorbent immunoassay (ic-ELISA) based on chicken IgY was developed and validated for the detection of enrofloxacin (ENR) residues in animal-derived food samples.The ENR immunogen and coating antigen were prepared by the active ester method and confirmed by UV spectroscopy for further detection and immunization into laying hens.IgY antibodies were extracted with PEG-6000 precipitation.The titer of anti-ENR IgY attained its peak (1∶32 000) after the fifth booster immunization.Checkerboard titration showed that 1∶64 000 dilution of anti-ENR IgY could give OD around 1.0 at 38 ng/mL ENR-OVA coating concentration.The IC50 of the ELISA for ENR was 18.207 ng/mL and the regression curve equation was y =0.891 1-0.016 5x (R2 =0.990).The study demonstrated that anti-ENR IgY antibody was feasible for the detection of ENR in animal-derived food samples.关键词
恩诺沙星/卵黄抗体/药物残留/酶联免疫吸附法Key words
enrofloxacin/IgY/antibiotic residues/indirect competitive enzyme-linked immunosorbent immunoassay (ic-ELISA)分类
农业科技引用本文复制引用
杨熠,孟坤杰,赵红琼,米克热木·沙衣布扎提,张小莺..酶联免疫吸附法检测动物源性食品中恩诺沙星残留量[J].食品科学,2017,38(8):239-243,5.基金项目
国家自然科学基金面上项目(31572556) （31572556）
2016年度国家外国专家局国文教专家聘请计划学校重点项目(X2015017) （X2015017）
中国-上合组织国家动物疫病与动物源性食品安全检测创新平台项目(2016E03012) （2016E03012）
