食品科学2017，Vol.38Issue(8)：251-257,7.DOI:10.7506/spkx1002-6630-201708039
人工接种温州蜜柑后链格孢霉毒素的产生及分布规律
Production and Distribution of Alternaria Mycotoxins in Satsuma Mandarin (Citrus unshiu Marc.) Artificially Inoculated with Alternaria alternate
摘要
Abstract
An utra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established using quick,easy,cheap,effective,rugged,and safe (QuEChERS) pre-treatment to investigate the production and distribution of five Alternaria mycotoxins including tentoxin (Ten),alternariol (AOH),alternariol monomethyl ether (AME),altenuene (ALT) and tenuazonic acid (TeA) in whole fruits,healthy pulp and healthy peel of citrus inoculated with Alternaria alternate.The results demonstrated that the contents of various Alternaria mycotoxins produced in whole fruits,healthy pulp and healthy peel changed differently with increasing lesion diameter.TeA,Ten,AME and AOH were detected in all tested parts of the fruit.Among these,the average concentration of TeA was the highest,ranging from 3.05 × 103 to 55.88 × 103 μg/kg and from 65.35 to 40.68 × 103 μg/kg in lesion and non-lesion areas,respectively.The concentrations of Ten,AME and AOH in the infected location were in the range of 69.16-373.94,22.63-1 395.82 and 8.18-689.19 μg/kg,respectively,while in the health location,the values were in the range of 0-67.56,0-195.96 and 0-301.91 μg/kg,respectively.ALT was detected in both healthy peel and infected whole fruits (up to 16.61 μg/kg) rather than healthy pulp.These results indicate that Alternaria mycotoxins produced in infected citrus can rapidly spread from the infected to the healthy parts,resulting in the accumulation of a large amount of mycotoxins in citrus.Therefore,we should pay more attention to this problem in fresh food processing and risk assessment to ensure consumer safety.关键词
温州蜜柑/链格孢霉毒素/超高效液相色谱-串联质谱/分布规律Key words
Satsuma mandarin/Alternaria mycotoxins/ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/distribution pattern分类
轻工纺织引用本文复制引用
蒋黎艳,龚蕾,盖智星,刘雁雨,马良,赵其阳,王成秋,焦必宁..人工接种温州蜜柑后链格孢霉毒素的产生及分布规律[J].食品科学,2017,38(8):251-257,7.基金项目
国家重点基础研究发展计划(973计划)项目(2013CB127803) （973计划）
国家自然科学基金青年科学基金项目(31301476) （31301476）
国家现代农业(柑桔)产业技术体系建设专项(CARS-27) （柑桔）
国家农产品质量安全风险评估重大专项(GJFP2015004 （）
GJFP2016004) （）
中央高校基本科研业务费专项(XDJK2013B035) （XDJK2013B035）
