食品科学2017，Vol.38Issue(8)：264-270,7.DOI:10.7506/spkx1002-6630-201708041
基于DNA核酸适配体序列的超灵敏比色法检测牛乳中的雌二醇
A Supersensitive Colorimetric Method Based on DNA Aptamers for Estradiol Detection in Milk
摘要
Abstract
A novel method was developed for the qualitative and quantitative determination of estradiol based on its specific recognition by the DNA aptamer of optimum length as well as the changes in the color and absorbance of colloidal gold before and after aggregation.In this work,colloidal gold particles were prepared and the 75-mer,35-mer and 22-mer estradiol-specific nucleic acid aptamers were designed for the ultrasensitive colorimetric detection of estradiol.The colodmetric method was based on the fact that the optimal concentration of sodium chloride leads to the aggregation of colloidal gold without the protection of the aptamer,while the colloidal gold does not aggregate under the protection of the aptamer.The results showed that the concentration of estradiol in the range of 13.6-54.4 pg/mL exhibited a good linear relationship with the absorbance ratio (A625 ~/A523 m) of colloidal gold at 625 nm and 523 nm with a limit of detection (LOD) of 2.7 pg/mL,under the condition of 30 mmol/L sodium chloride and 30 nmol/L 35-mer nucleic acid aptamer.The developed colorimetric sensor had the advantages of high specificity,stability and reproducibility.The practicality of this proposed method was further confirmed through the detection of estradiol in milk samples with LOD of 13.6 pg/mL.Therefore,this colorimetric method will be useful for the rapid and sensitive detection of estradiol in milk products.关键词
雌二醇/胶体金/DNA核酸适配体/比色法Key words
estradiol/colloidal gold/DNA aptamer/colorimetry分类
轻工纺织引用本文复制引用
付田,曹锦轩,潘道东,程克文,曾小群,吴振,孙杨赢..基于DNA核酸适配体序列的超灵敏比色法检测牛乳中的雌二醇[J].食品科学,2017,38(8):264-270,7.基金项目
“十二五”国家科技支撑计划项目(2015BAD17B02-3) （2015BAD17B02-3）
浙江省攻关项目(2014C02020) （2014C02020）
