食品科学2017，Vol.38Issue(8)：303-307,5.DOI:10.7506/spkx1002-6630-201708047
狐狸和貉子动物源性成分双重PCR鉴别
Duplex PCR Distinction of Food Ingredients Derived from Alopex lagopus and Nyctereutes procyonoides
摘要
Abstract
Two sets of specific primers were designed based on the conserved mitochondrial gene sequences of Alopex lagopus and Nyctereutes procyonoides and used to establish a PCR method to detect ingredients derived from A.lagopus and N.procyonoides in commercial meat products.Regular and clear A.lagopus-and N.procyonoides-specific bands were amplified simultaneously by duplex PCR.The sensitivities of the A.lagopus and N.procyonoides primers were respectively 10 pg and 1 pg in separate polymerase chain reactions,respectively.By contrast,the sensitivities of the A.lagopus and N.procyonoides primers were respectively 100 pg and 10 pg in duplex PCR due to the competition of two sets of primers.关键词
狐狸/貉子/双重PCR/肉类掺假Key words
Alopex lagopus/Nyctereutes procyonoides/duplex PCR/meat adulteration分类
轻工纺织引用本文复制引用
杜利强,章晶晶,张涛,齐艳玲,李顺才,李岩,周巍,张岩..狐狸和貉子动物源性成分双重PCR鉴别[J].食品科学,2017,38(8):303-307,5.基金项目
河北省科技厅重点研发计划项目(16275502D) （16275502D）
河北省高等学校科学技术研究青年基金项目(QN2014159) （QN2014159）
