食品与发酵工业2017，Vol.43Issue(4)：15-21,7.DOI:10.13995/j.cnki.11-1802/ts.201704003
ClassⅡ细菌素乳酸菌的筛选与定性及群体感应系统鉴定
Identification and screening of Class Ⅱ bacteriocins of lactic acid bacteria and quorum sensing system
吴林昊 1钱宇 1汪慧超 1薛秀恒1
作者信息
- 1. 安徽农业大学茶与食品科技学院,安徽农产品加工工程实验室,安徽合肥,230036
- 折叠
摘要
Abstract
The lactic acid bacteria with broad-spectrum antimicrobial effect of Class Ⅱ bacteriocin quorum sensing system were screened and identified.After exclusion of hydrogen peroxide and organic acid interference and treatment with trypsin,pepsin and proteinase K,lactic acid bacteria secreting bacteriocin with broad-spectrum antibacterial effect was screened by Oxford cup method.The minimal inhibitory concentration was detected by assay on minimal inhibitory activity.Phylogenetic tree was constructed based on 16S rDNA homology analysis.The primers specific for five operon plnEFI,plnJKLR,plnGHSTUV,plnABCD,and plnMNOP of the class Ⅱ bacteriocins were designed.Class Ⅱ bacteriocin genes of quorum sensing system were identified by PCR and electrophoresis.The molecular size of bacteriocin was determined by UPLC and urea-sds-page.L-4 was a kind of gram positive bacteria with broad spectrum antimicrobial activity.The diameters of inhibition zones for indicator bacteria were (18.83 ± 0.39) mm and (19.96 -±0.49) mm,with the minimal inhibitory concentrations of 55.68 μg/mL and 57.31 μg/mL.L-4 had Class Ⅱ bacteriocin gene of quorum sensing system.It could secrete a bacteriocin with molecular weight of 10 ku in the molecular range of Class Ⅱ bacteriocin.L-4 was a strain of Lactobacillus plantarum secreting 10 ku of Class Ⅱ bacteriocin with broad-spectrum antimicrobial activity.关键词
乳酸菌/细菌素/16S rDNA鉴定/系统进化树/Class Ⅱ细菌素群体感应系统Key words
lactic acid bacteria/bacteriocin/16S rDNA identification/phylogenetic tree/Class Ⅱ bacteriocin quorum sensing system引用本文复制引用
吴林昊,钱宇,汪慧超,薛秀恒..ClassⅡ细菌素乳酸菌的筛选与定性及群体感应系统鉴定[J].食品与发酵工业,2017,43(4):15-21,7.
