食品与发酵工业2017，Vol.43Issue(4)：34-40,7.DOI:10.13995/j.cnki.11-1802/ts.201704006
混合糖发酵产油脂酵母菌的筛选及脂肪酸组成分析
Screening of oil-producing yeast strains by co-fermentation of glucose and xylose and lipid-acid component analysis
摘要
Abstract
In this paper,the strain YP-32 which could use mixed sugar of glucose and xylose as carbon source to produce lipid were screened from 12 wild yeast strains from the soil of Hainan province.The biomass,lipid concentration and lipid content of the strain reached 22.33 g/L,13.39 g/L and 60.42％ respectively by fermenting with 2:1 of glucose and xylose as carbon source.Simultaneously,lipid-acid component was analyzed.There was almost no difference in lipid-acids component by using different mixed sugar ratios as carbon source.Results showed that C16 and C18 series were main fatty acids.Oleic acid content was the highest,palmitic acid and stearic acid were next in all samples and the sum of them accounted for over 90％ of total content of lipid acids.The lipid component of this strain was similar to plant oil and could be used as biodiesel source.The strain was primarily identified as Lipomyces orientalis by the morphology feature,26S rDNA D1/D2 and ITS domain sequence analysis.The strain is a new record for distribution in our country.关键词
产油脂酵母菌/筛选/混合糖发酵/脂肪酸组成/鉴定/海南省Key words
oleaginous yeast/screening/mixed sugar fermentation/lipid-acid component/identification/Hainan province引用本文复制引用
张艳平,白新鹏,郭书贤,王冬梅..混合糖发酵产油脂酵母菌的筛选及脂肪酸组成分析[J].食品与发酵工业,2017,43(4):34-40,7.基金项目
河南省基础与前沿技术项目(132300410087) （132300410087）
河南省基础与前沿技术研究项目(102300410059) （102300410059）
